The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 12, 1998
Filed:
Jun. 21, 1995
Jean-Louis Drocourt, Yerres, FR;
Jean-Gerard Guillet, Vanves, FR;
Warren Groner, Great-Neck, NY (US);
Chemunex, Maisons-Alfort, FR;
Abstract
Apparatus and process capable of rapid detection and counting of rarely occuring mammalian cells in blood and other tissues which have been labeled with a fluorescent dye. The process includes: scanning a solid support on which a specimen potentially containing fluorescent cells has been deposited, with an incident beam from a laser, forming a laser spot on the solid support, the laser spot being substantially greater than the cells to be detected, the laser spot size being between 15 and 30 .mu.m; and simultaneously: detecting the resultant fluorescent light at least at one wavelength; establishing a set of correlated-features by a line-to-line correlation of individual features; comparing the correlated-features on each pair of adjacent lines in time synchrony, at least at two different wavelengths .lambda..sub.1 and .lambda..sub.2 ; making a size discrimination of retained events and selecting those having a size corresponding to the type of cells searched; determining if, for retained events after size discrimination, the events energy profile in three dimensions is within predetermined Gaussian shape criteria; and counting the remaining events to determine and to count exclusively the fluorescent cells present on the solid support.