The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 1998
Filed:
Feb. 07, 1995
Applicant:
Inventors:
Christopher Robert Bebbington, Berkshire, GB;
Geoffrey Thomas Yarranton, Berkshire, GB;
Assignee:
Celltech Therapeutics Limited, Berkshire, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
4351723 ; 435326 ; 4353721 ; 435 691 ; 4353201 ;
Abstract
There is described a method for obtaining a eukaryotic cell containing in its DNA multiple copies of a GS gene, comprising: transforming a eukaryotic glutamine auxotroph with a GS gene; selecting transformant cells containing the GS gene; and culturing the selected transformant cells in a medium which lacks glutamine or in which the amount of glutamine is progressively depleted, the GS gene being of a character such that, or the conditions employed during the culturing step being such that, the GS gene is so weakly transcribed that the cells in which the GS gene has been amplified are selected.