Method and device for specific binding assay

Abstract

The present invention provides a general purpose specific binding assay method which has the advantages of highly accurate and quick measurements which exclude the effects of various factors that decrease reliability of the measured values, such as non-specific reactants in test samples, assay conditions and inactivation and the like changes in the activity of reagents. The present invention is further drawn to a specific binding assay device suitable for the practice thereof. The binding assay of the present invention is achieved by allowing a signal substance generator which takes part in a specific binding reaction and generates a signal substance, together with a liquid sample, to flow through a predetermined channel in a predetermined direction, thereby effecting generation of the specific binding reaction of a substance to be assayed to form a distribution of the signal substance generator in the channel in response to the concentration of the substance to be assayed, allowing the signal substance generator distributed in the channel to generate the signal substance, detecting the generated signal substance by a plurality of detection means arranged at different positions in the liquid flow direction, and arithmetically processing the plural detection results to minimize influence of other factors than the concentration of the substance to be assayed upon the assay result.