The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 03, 1998
Filed:
Sep. 16, 1996
Abraham Oommen, Lincoln, NE (US);
Steve Roemer, Lincoln, NE (US);
Li-Cor, Inc., Lincoln, NE (US);
Abstract
The present invention provides a novel method for labeling and sequencing nucleic acid molecules, particularly DNA molecules in which an internally labeled, partially extended primer is elongated in a cycled primer extension reaction. An unlabeled DNA primer is contacted with a DNA template in the presence of suboptimal amounts of four dNTPs, one of which is labeled with a detectable marker which may be a fluorescent or visible fluorophor, and infared fluorophor or a radioactive label. This small, labeled primer extension product is then transferred to a new reaction where chain terminated primer extension products for DNA analysis are prepared.