The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 17, 1998
Filed:
Jun. 01, 1995
Jonathan S Dordick, Iowa City, IA (US);
Vikram M Paradkar, Madison, WI (US);
University of Iowa Research Foundation, Iowa City, IA (US);
Biotechnology Research & Development Corp., Peoria, IL (US);
Abstract
Organic enzyme solutions comprise an ion pair complex of an enzyme and a surfactant, and an organic solvent in which the enzyme-surfactant ion pair is dissolved. The solution has catalytic activity at least an order of magnitude greater than a suspension of an equal amount of the enzyme and organic solvent without ion pair complexes. The enzyme is preferably a hydrolase with an acyl transferase activity and the surfactant is Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) at low concentrations. The organic enzyme solution may be made by obtaining an aqueous solution of an enzyme at a pH of maximal enzyme activity, adding an ionic surfactant to the aqueous solution in an amount sufficient to form an ion pair, and extracting the enzyme-surfactant ion pair into an organic solvent phase without substantial formation of reversed micelles, to produce an anhydrous organic enzyme solution comprising the enzyme-surfactant ion pair dissolved in the organic solvent such that the enzyme retains native structure and substantial catalytic activity. Catalytic solutions according to the invention can be used to catalyze peptide synthesis from acyl donors and nucleophiles.