The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 03, 1998

Filed:

Jul. 31, 1995
Applicant:
Inventors:

Helena Tuompo, Espoo, FI;

Helja Glasin, Espoo, FI;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M / ;
U.S. Cl.
CPC ...
435 29 ; 435-4 ; 435 30 ; 435 34 ; 435 38 ; 435 39 ; 435810 ;
Abstract

A method for determining the presence of microorganisms in a liquid sample. The liquid sample is first filtered through a filter having a pore size which is small enough to prevent passage of microorganisms through the filter but large enough to permit passage of any free reducing agents present in the sample whereby microorganisms present in the sample are retained on the filter. A chromogenic reagent having an oxidation potential such that the reagent can be reduced by microbial dehydrogenase and selected such that reduction of the chromogenic reagent yields a visibly colored product is then passed through the filter, and the filter is monitored for the formation of a visibly colored product indicative of the presence of microorganisms in the sample. The method can be practiced with the aid of a kit which includes a filter apparatus, a test solution for visualization of microorganisms and optionally a solution of a non-ionic alkyl glucoside-type detergent and a chaotropic agent for visualization only gram-negative bacteria.


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