The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 27, 1998

Filed:

Nov. 22, 1994
Applicant:
Inventors:

Su May Yu, Taipei, TW;

Li Fei Liu, Taipei, TW;

Ming Tsair Chan, Taipei, TW;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 435 697 ; 435 698 ; 435 701 ; 4351723 ; 435410 ; 435420 ; 536 231 ; 536 236 ; 536 241 ; 935 35 ; 935 60 ; 935 67 ;
Abstract

The present invention is directed to a method for producing a gene product by expressing a gene encoding the gene product in angiosperm host cells, comprising the steps of constructing a vector expressible in angiosperm host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product; transforming a compatible angiosperm host cell with the vector; cultivating the resultant transformant host cell; subjecting the cultivated transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of the gene under the control of the promoter region; and recovering the expressed gene product. The present invention also is directed to a method for the production of a transgenic plant of rice crop comprising the steps of infecting an immature embryo of rice crop with the genus Agrobacterium for transformation; co-culturing the infected embryo with a dicot suspension culture during the step of transformation; allowing the transformed embryo to grow into a callus in a selective medium comprising a sufficient amount of a plant growth hormone for the growth of rice crop; and allowing the cultured callus to regenerate root and shoot in a regeneration medium comprising a pre-determined amount of nutrients for the growth of rice crop. The invention is further directed to a transformed rice plant made by methods of this invention.


Find Patent Forward Citations

Loading…