The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 20, 1998

Filed:

Feb. 03, 1995
Applicant:
Inventors:

Shane Crawford Weber, Woodbridge, CT (US);

Arthur Herman Herz, Rochester, NY (US);

Assignee:

Eastman Kodak Company, Rochester, NY (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K / ;
U.S. Cl.
CPC ...
530356 ; 530412 ; 435 691 ;
Abstract

A method for preparing and purifying collagen-type polypeptides (or biopolymers) has been developed. The polypeptides are prepared by recombinant DNA technology using yeast host cells. Once expressed in the host cell culture, the polypeptide is purified by a series of centrifugations, isoprecipitation, ion exchange (or other chromatography), and diafiltration steps. Treatments with a protease and a calcium salt are also carried out at some point. It is also desirable to diafilter the purified fractions from the last chromatography to exchange ions which make the resulting polypeptide more compatible with its intended use.


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