The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 13, 1998
Filed:
Feb. 07, 1996
Christopher D Wilsey, Carmel, IN (US);
Helmut Freitag, Weinheim, DE;
Boehringer Mannheim Corporation, Indianapolis, IN (US);
Abstract
A new reagent and methods for measuring the concentration of (or detecting the presence of) an analyte in a sample. The reagent includes a phenazine-containing compound and an enzyme. The phenazine-containing compound must be of sufficient type to form a semiquinoid (the color indicator) by reaction involving the enzyme, analyte, and phenazine-containing compound. Importantly, the phenazine-containing compound must be in sufficient amount to correlate the concentration of semiquinoid to the concentration of analyte in the sample or to detect the presence of the analyte in the sample. The reagent may further include a buffer and a surfactant. The reagent may be incorporated into a film and may be provided in kit form. The methods for measuring the amount of (or detecting the presence of) an analyte in a sample importantly include spectrophotometric measurement (or detection) of the semiquinoid indicator at wavelengths greater than about 580 nanometers, which reduces interferences due to the presence of hemoglobin, bilirubin, and turbidity. Further, these assay methods importantly involve short incubation periods of less than about one minute for test samples measuring (or detecting) spectrophotometric absorbance (assays performed on solutions) and less than about 1.5 minutes for test samples measuring (or detecting) spectrophotometric reflectance or transmittance (assays performed on films).