Multi-biosensor for gpt and got activity

Abstract

Multi-biosensor in which only one enzyme is used for handy and simultaneous measurement of GPT and GOT activities, and a method for fabricating the multi-biosensor, are disclosed, the biosensor including an insulating substrate; a reference electrode formed on the insulating substrate; first and second working electrodes formed on one side of the insulating substrate having the reference electrode formed thereon; third and fourth working electrodes formed on the other side of the insulating substrate having the reference electrode formed thereon opposite to the first and second working electrodes centered on the reference electrode; first, second, third and fourth carbon paste layers formed on the first, second, third and fourth working electrodes respectively; first and second enzyme/polymer carbon paste layers formed on the first and second carbon paste layers respectively; first and second non-enzyme/polymer carbon paste layers formed on the third and fourth carbon paste layers respectively; an amino acid/polymer paste layer for making an enzymatic reaction with GPT formed extended to the first enzyme/polymer carbon paste layer and the first non-enzyme/polymer carbon paste layer; and, an amino acid/polymer paste layer for making an enzymatic reaction with GOT formed extended to the second enzyme/polymer carbon paste layer and the second non-enzyme/polymer carbon paste layer.