The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 23, 1997

Filed:

Oct. 08, 1993
Applicant:
Inventors:

Diana K Sheiness, Bothell, WA (US);

Trevor H Adams, Buckinghamshire, GB;

Michael R Stamm, Bothell, WA (US);

Gerard A Cangelosi, Seattle, WA (US);

Theresa B Britschgi, Seattle, WA (US);

Connie K Dix, Arlington, WA (US);

Assignee:

Becton Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 536 2432 ;
Abstract

The present invention relates, in general, to methods for selectively detecting a prokaryotic microorganism and a eukaryotic microorganism in a single complex biological sample wherein the cells of such microorganisms are lysed by combining the sample with a lysis solution and contacting the nucleic acid released from the microorganisms with selective nucleic acid probes through hybridization techniques. Further provided are methods which permit the detection of a Gram-positive bacterium with at least one other microorganism selected from the group consisting of yeast, protozoa, mycoplasma and Gram-negative bacteria in a single complex biological sample. In other aspects of the invention, the methods disclosed permit the detection of microorganisms associated with vaginal disorders.


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