The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 16, 1997

Filed:

Jun. 02, 1995
Applicant:
Inventors:

Marcia K Wolf, Silver Spring, MD (US);

Frederick J Cassels, Ellicott City, MD (US);

Brian A Bell, Clarksburg, MD (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 435 712 ; 43525233 ;
Abstract

Production of proteins in bacteria containing DNA sequences encoding proteins under the control of a temperature-regulated promotor is improved by growing the organisms at temperatures of less than 35.degree. C. until the late logarithmic phase. Thereafter the temperature may be raised to 36.degree. C. to 39.degree. C. Antigens produced by the method of the invention may be used as vaccines, as means for measuring efficacy of vaccines, as probes to detect antigens from clinical samples and for biochemical characterization of antigens.


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