The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 11, 1997

Filed:

Nov. 09, 1994
Applicant:
Inventor:

Abul K Ekramoddoullah, Victoria, CA;

Assignee:

Forestry Canada, Hull, CA;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ; C07K / ; C07K / ; A01H / ;
U.S. Cl.
CPC ...
435-71 ; 435-792 ; 435975 ; 5303871 ; 530350 ; 800200 ; 800D / ;
Abstract

A method of determining frost hardiness of a conifer seedling, which comprises detecting an amount of a cold protein in tissue of said conifer, the cold protein being a protein of approximately 19 kD that increase significantly in amount in the conifer during fall season (autumnal) months and that imparts frost hardiness to the seedling, and then assessing frost hardiness based on said detected amount. In the case of Pinus lambertiana, the cold protein includes the N-terminal sequence: Val--Ser--Gly--Thr--Ser--Ser--Thr--Glu--Glu--Val--Val--Gln--Asn--Glu--Ala- -Arg--Arg--Leu--Trp--Asn �SEQ ID NO:1!; and in the case of Pinus monticola, the cold protein includes the N-terminal sequence: Val--Ser--Gly--Thr--Ser--Ser--Thr--Glu--Glu--Val--Val--Gln--Val--Glu--Ala- -Arg--Arg--Leu--Trp--Asn--Ala--Thr--Thr--Lys--Asp �SEQ ID NO:3!. The invention also relates to conjugates and antibodies used in the method.


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