The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 04, 1997
Filed:
Jun. 07, 1995
Nrusingha C Mishra, Germantown, MD (US);
Hossein S Khorshidi, Vienna, VA (US);
Yuxiang Gan, Bethesda, MD (US);
Pam Szweda, Cleveland Heights, OH (US);
Jay George, Gaithersburg, MD (US);
Oncor, Inc., Gaithersburg, MD (US);
Abstract
Modified pyrimidine bases and nucleotides containing a linker arm and a detectable reporter group for nucleic acid labeling. The modified nucleotides have the structure: ##STR1## wherein: -R.sub.1 is --H or --OH; -R.sub.2 and -R.sub.3 are independently --H, --OH, monophosphate, diphosphate, triphosphate, thio analogs of mono-, di-, or triphosphates, --O-- attached to a reactive phosphorous-containing group or --O-- protected by a blocking group; -A and -B are independently --H, an electron donating group, an electron withdrawing group, C.sub.1 -C.sub.4 alkyl, halogen, nitro, --COOH, amino, substituted amino, cyano, --CONH, --CSNH, --COOR.sub.4 ##STR2## --CSOR.sub.4, --COSR.sub.4, --SR.sub.4, --COR.sub.4, --CH.sub.2 NHR.sub.4, -R.sub.4 C.dbd.CR.sub.5 H or --C.tbd.C--R.sub.4 where R.sub.4 and R.sub.5 are independently H, an electron donating group, an electron withdrawing group, C.sub.1 -C.sub.4 alkyl, halogen, nitro, COOH, amino, substituted amino, cyano, CONH or CSNH; -X- is --NH--, --NHNH--, --O--, --S--, ##STR3## or --CH.sub.2 --; Rep is a detectable reporter group; and -L- is --(CH.sub.2).sub.n -(T).sub.q --(CH.sub.2).sub.m Z.sub.1 or --(CH.sub.2).sub.n -(T).sub.q --(CH.sub.2).sub.m -Z.sub.2 --(CH.sub.2).sub.p Z.sub.1 where n, m and p are each independently an integer from 1 to 20 and q is 1 or 2, -T- is --O--, --S--, ##STR4## --NH-- or a heterocyclic structure containing one or more Group Va or VIa elements as a ring atom, Z.sub.1 is a covalent bond, --NH--, --CO--, --O--, --S-- or ##STR5## and -Z.sub.2 -- is --NH--, --NHCO--, --NHCS--, --CONH--, --CSNH--, --O--, --S-- or ##STR6##