The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 21, 1997
Filed:
Aug. 09, 1996
Applicant:
Inventors:
Theo Nikiforov, Baltimore, MD (US);
Jonathan Karn, Little Shelord, GB;
Philip Goelet, Cockeysville, MD (US);
Assignee:
Molecular Tool, Inc., Baltimore, MD (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435-6 ; 435-5 ; 435 911 ; 435 912 ; 435810 ; 436501 ; 536 241 ; 536 243 ; 536 2431 ; 536 2432 ; 536 2433 ; 935 77 ; 935 78 ;
Abstract
A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.