The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 1997
Filed:
Nov. 10, 1994
Takashi Matsuzaki, Hiroshima, JP;
Mutsumi Inamatsu, Higashi-Hiroshima-shi, Hiroshima, JP;
Katsutoshi Yoshizato, Hiroshima, JP;
Research Development Corportion of Japan, Kawaguchi, JP;
Other;
Abstract
This invention provides a method for preparing hybridomas, which include the steps of: quickly and deeply freezing hair-bulbs of follicle isolated from a human or an animal of species A; immunizing an animal of species B by subcutaneously administering the hair-bulbs after being thawed out to the animal; re-immunizing the animal by intravenously and intraperitoneally administering liquid and solid components of hair-bulbs obtained by centrifugation of minced thawed hair-bulbs to the animal; fusing spleen cells or lymphoid cells isolated from the immunized animal with myeloma cells derived from an animal of species B or C, and cloning fusion cells which produce antibodies capable of specifically binding to the hair follicle of human or animal of species A. The hybridomas prepared by the above methods can produce monoclonal antibodies specifically recognizing hair follicle tissues, and these antibodies enable functional analysis of hair follicle in addition to development of new diagnostic and therapeutic means for abnormal hair development, alopecia, etc.