The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 30, 1997
Filed:
Mar. 06, 1996
Steven K Striepeke, Sebastopol, CA (US);
Peter Edridge, Fairfax, CA (US);
Christopher M Starr, Sonoma, CA (US);
John C Klock, Nicasio, CA (US);
Glyko, Inc., Novato, CA (US);
Abstract
An apparatus for fluorescence of a biological sample includes a light illumination source, an ambient light-excluding optical chamber, a sample holder, a two-dimensional array charge coupled device ('CCD') sensor providing for on-chip signal integration of each pixel of the array, and an analog/digital converter for digitizing the CCD signal. Signal acquisition time from the array is varied so that the integrated signal from each pixel is below its saturation level. A darkfield value for each pixel is acquired and subtracted from the illuminated signal from that pixel so as to yield a darkfield corrected signal. The fluorescence value for each pixel can be compensated for variations in excitation energy reaching different sample areas. Image readout is through a single channel output.