The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 26, 1997
Filed:
Jun. 06, 1996
William K Boss, Jr, Cedar Grove, NJ (US);
Isolagen Technologies, Inc., West Orange, NJ (US);
Abstract
The application concerns a device for repairing a dermal defect in a subject comprising: a) a hypodermic syringe having a syringe chamber, a piston disposed therein, and an orifice communicating with the chamber; b) a suspension comprising: 1) dermal fibroblasts derived from the subject, said fibroblasts being substantially free of cells other than fibroblasts and substantially free of proteins that are immunogenic in the subject, and 2) a pharmaceutically acceptable carrier solution, said suspension being disposed in the chamber; and c) a hypodermic needle affixed to the orifice. The application further concerns a method of making the above-identified device, comprising the steps of: a) biopsying the dermis of the subject; b) passaging the dermal fibroblasts from the dermal biopsy in a culture medium comprising between 0.5% and 20% non-human serum, so as to provide dermal fibroblasts substantially free of cells other than fibroblasts; c) incubating the passaged dermal fibroblasts in a serum-free medium for at least 6 hours at between about 30.degree. C. and about 40.degree. C.; d) exposing the incubated fibroblasts to a proteolytic enzyme so as to suspend the fibroblasts; and e) adding the suspended fibroblasts to a pharmaceutically acceptable carrier solution so that a suspension is formed and placing said suspension into the syringe chamber.