The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 19, 1997

Filed:

Aug. 26, 1994
Applicant:
Inventors:

Jeroen Van Rie, Eeklo, BE;

Stefan Jansens, Gent, BE;

Marnix Peferoen, Nevele, BE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01H / ; C12N / ; C07K / ;
U.S. Cl.
CPC ...
800205 ; 800250 ; 800D / ; 514 12 ; 536 2371 ; 4351723 ;
Abstract

New modified Cry proteins, particularly modified CryIIIA proteins, having significantly altered toxicity, and DNA sequences encoding these proteins, are designed. Analysis of solvent-accessible amino acid positions in domain II of the CryIIIA protein by alanine-scanning mutagenesis identified individual amino acids involved in corn rootworm toxicity. Random replacement of these amino acids identifies modified proteins with improved toxicity. A combination of all or most thus identified improved amino acids in a single protein yields modified CryIIIA proteins with significantly improved toxicity. Particularly regions protruding from the Cry molecule and located at the apex of the Cry proteins, were identified as involved in toxicity.


Find Patent Forward Citations

Loading…