The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 19, 1997
Filed:
May. 19, 1995
Applicant:
Inventors:
Vito Quaranta, La Jolla, CA (US);
Marketta Hormia, Helsinki, FI;
Assignee:
Desmos, Inc., San Diego, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C07K / ;
U.S. Cl.
CPC ...
435375 ; 435325 ; 514-2 ; 530350 ; 530827 ; 530828 ; 530834 ;
Abstract
A method for growing epithelial cells in vitro using soluble proteins secreted by 804G and NBT-II rat bladder carcinoma cells. These proteins stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The protein is purified from conditioned medium by immunoaffinity chromatography using a monoclonal antibody generated against the protein. The purification of these proteins may be facilitated by culturing the cells under low serum or serum free conditions.