The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 12, 1997
Filed:
Oct. 05, 1994
Applicant:
Inventors:
Ronald L Crawford, Moscow, ID (US);
Yongxiang Gu, Moscow, ID (US);
Roger A Korus, Moscow, ID (US);
David B Knaebel, Moscow, ID (US);
Assignee:
Idaho Research Foundation, Inc., Moscow, ID (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12N / ;
U.S. Cl.
CPC ...
435-4 ; 435 25 ; 435 42 ; 435 46 ; 435810 ; 435822 ; 435829 ; 435849 ; 435174 ; 435177 ; 435179 ; 435180 ; 435325 ; 435349 ; 435348 ; 435419 ; 435365 ; 435367 ; 435358 ;
Abstract
Methods and compositions are provided for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and other phenoxy ether compounds. The phenoxy ether bond of 2,4-D is enzymatically cleaved by 2,4-D .alpha.-ketoglutarate dioxygenase to form 2,4-dichlorophenol, which is assayed by the 4-aminoantipyrine method. The enzyme is supplied in a dried form, preferably immobilized on a solid support, and is stable at room temperature for several months even in a highly impure state, e.g., crude cell extracts or dried cells.