The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 15, 1997
Filed:
Nov. 21, 1994
Tsunetomo Takei, Tokyo, JP;
Toshimitsu Aiba, Tokyo, JP;
Kaoru Sakai, Tokyo, JP;
Tetsuro Fujiwara, Morioka, JP;
Tokyo Tanabe Company Limited, Tokyo, JP;
Abstract
The present invention is directed to a purification method for hydrophobic polypeptides by using high performance liquid chromatography, characterized by using a mixed solvent as a moving phase, in which the portion of trifluoroacetic acid is from 3 to 10% by volume, and polyvinyl alcohol-based column filler in the said high performance liquid chromatography. The method according to the present invention is useful as a purification method for hydrophobic polypeptides in view of the fact that the pulmonary surfactant, which is prepared from the hydrophobic polypeptide purified according to the method of the present invention, shows better surface active property than the hydrophobic polypeptides purified by customary methods for the purification.