The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 08, 1997

Filed:

Aug. 18, 1995
Applicant:
Inventors:

Stanley R Bouma, Grayslake, IL (US);

Ronald A Coules, Barrington, IL (US);

Julian Gordon, Lake Bluff, IL (US);

Eric B Shain, Glencoe, IL (US);

Natalie A Solomon, Buffalo Grove, IL (US);

Peter Zaun, Libertyville, IL (US);

Assignee:

Abbott Laboratories, Abbott Park, IL (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12M / ; C12P / ;
U.S. Cl.
CPC ...
422 681 ; 422 50 ; 422 52 ; 422 55 ; 422 57 ; 422 58 ; 422 61 ; 422 63 ; 422 69 ; 435-6 ; 435 911 ; 435 912 ; 4352831 ; 4352871 ; 4352872 ; 4352873 ; 4352881 ; 4352882 ; 4352887 ; 4352891 ; 4352901 ; 4352904 ; 4352931 ; 4353041 ; 435810 ; 536 231 ; 536 241 ; 536 243 ; 536 2431 ; 536 2432 ; 536 2433 ; 935 77 ; 935 78 ; 935 88 ;
Abstract

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.


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