The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 1997
Filed:
Oct. 21, 1994
Michael Pfreundschuh, Homburg/Saar, DE;
Biotest Pharma GmbH, Dreieich, DE;
Abstract
The present invention relates to a method for the selective preparation of hybridoma cell lines which produce a murine monoclonal antibody (MAK) of the IgGl class with a high capacity for including NK cell-relate cytotoxicity against human CD16 antigen by co-culturing the hybridoma cells in the selection medium with unstimulated human NK (natural killer) cells. The invention furthermore relates to a cell line A9 of the DSM deposit number ACC 2148 obtainable in this manner, and a MAK obtainable thereform, and a method for the preparation of bispecific MAK's by the fusion of an anti-CD30 cell line HRS-3 with hybridoma cell lines, which is obtained by the above-mentioned selection process, expecially with the A9 line of DSM deposit number ACC 2148 obtaining especially the bispecific MAK of the HRS-3/A9 cell line with the DSM deposit number ACC 2142. These bispecific MAK's are suitable for the treatment and for the shrinking of established human tumors, especially human Hodgkin's tumors.