The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 1997
Filed:
Sep. 19, 1994
Applicant:
Inventors:
Edward Hinchliffe, Burton Joyce, GB;
Simon A Chinery, Bingham, GB;
Assignee:
Delta Biotechnology Limited, Nottingham, GB;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ; C12P / ;
U.S. Cl.
CPC ...
4353201 ; 435 691 ; 435 692 ; 435 711 ; 435171 ; 4351721 ; 4351723 ; 4352551 ; 935-6 ; 935-9 ; 935 22 ; 935 33 ; 935 57 ; 935 59 ; 935 60 ; 935 61 ; 935 66 ; 935 69 ;
Abstract
A 2 .mu.m plasmid vector for transforming yeast, especially brewing yeast, much which comprises a DNA sequence allowing propagation of the plasmid in bacteria, two copies of the 74 base pair FLP recombination site of the 2 .mu.m plasmid in direct orientation and a DNA sequence coding for a protein or peptide of interest. The plasmid is so constructed that in yeast the bacterial DNA sequence is between the two recombination sites which are in direct orientation and is spontaneously lost following recombination. A 'gene of interest' is preferable inserted at the SnaBI site.