The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 1997
Filed:
Dec. 13, 1994
Hernan R Campana, Habana, CU;
Bianca M Garcia Garcia, Habana, CU;
Julio M Delgado Boada, Habana, CU;
Jose A Cremata Alvarez, Habana, CU;
Maria E Gonzalez Martinez, Habana, CU;
Emilio M Clark, Habana, CU;
Dania M Curbelo, Habana, CU;
Luis S Herrera Martinez, Habana, CU;
Manuel R Raices Perez-Castaneda, Habana, CU;
Efrain R Jiminez, Habana, CU;
Rossana G Fernandez, Habana, CU;
Vivian M Cordova, Habana, CU;
Carlos F Patron, Habana, CU;
Centro de Ingenieria Genetica y Biotecnologia, Havana, CU;
Abstract
A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.