The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 10, 1997
Filed:
Apr. 21, 1993
Applicant:
Inventor:
Elizabeth M Van Cott, Salem, MA (US);
Assignee:
New England Biolabs, Inc., Beverly, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
536 232 ; 435-6 ; 4351723 ; 4352523 ; 435243 ; 4353201 ; 336 232 ; 935-1 ; 935-6 ; 935 22 ; 935 33 ; 935 52 ; 935 66 ;
Abstract
The present invention is directed to a method for cloning and producing the SfiI restriction endonuclease by 1) introducing the restriction endonuclease gene from S. fimbriatus into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SfiI restriction endonuclease activity; and 3) purifying the SfiI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SfiI restriction endonuclease activity.