The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 22, 1997
Filed:
Sep. 13, 1994
Applicant:
Inventors:
Tobias E Ekeze, Rocheser, NY (US);
John W Backus, Williamson, NY (US);
David J Sharkey, Rochester, NY (US);
Richard C Sutton, Rocheser, NY (US);
JoAnne H Kerschner, Rochester, NY (US);
Assignee:
Johnson & Johnson Clinical Diagnostics, Inc., Rochester, NY (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12P / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435-5 ; 435 912 ; 435 911 ; 536 243 ; 536 2432 ; 536 2433 ; 424 941 ;
Abstract
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.