The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 22, 1997

Filed:

Jun. 29, 1994
Applicant:
Inventors:

Paul R Selvin, Berkeley, CA (US);

John Hearst, Berkeley, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435-6 ; 540-1 ; 540450 ; 540451 ; 540452 ; 540455 ; 540465 ; 540467 ; 540476 ; 546-1 ; 546-2 ; 546 22 ; 546 23 ; 546 24 ; 546 26 ; 546 61 ; 549-1 ; 549-3 ; 549-5 ; 549 13 ; 549 23 ; 549200 ; 549204 ; 549206 ; 549218 ; 549220 ; 549263 ; 549273 ; 549283 ; 435-71 ; 435-72 ; 435810 ; 436501 ; 436546 ; 436536 ; 436 56 ; 436 63 ; 436 73 ; 436 81 ; 436 82 ; 436164 ; 436172 ; 436800 ; 436805 ; 530300 ; 530350 ; 536 221 ; 536 231 ; 536 243 ; 536 2431 ; 536 2432 ; 536 2433 ; 935 77 ; 935 78 ;
Abstract

The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc. The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.


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