The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 18, 1997
Filed:
Dec. 01, 1994
Bogi Andersen, La Jolla, CA (US);
Richard V Pearse, San Diego, CA (US);
Peter N Schlegel, New York, NY (US);
Michael G Rosenfeld, San Diego, CA (US);
C Wayne Bardin, New York, NY (US);
The Population Council, Center for Biomedical Research, New York, NY (US);
Abstract
Disclosed is a POU transcription factor, Sprm-1 and analogs thereof, DNA molecules encoding the Sprm-1 proteins, and chimeric constructs, vectors and host cells containing the DNA molecules. Also disclosed are methods to identify putative male contraceptive agents. One involves the steps of isolating a DNA regulatory region responsive to Sprm-1, stably transforming a heterologous cell line with a first chimeric DNA construct containing a constitutive promoter capable of functioning in the cell line operably linked to a Sprm-1 DNA and a second chimeric DNA construct containing the DNA regulatory region operably linked to a reporter gene. The thus-transformed cell line is cultural in media containing the compound so that the thus-cultured cell line can be assayed to determine the Sprm-1 inhibitory activity of the compound. The above method can be modified to evaluate the Sprm-1 inhibitory activity of DNA molecules of interest, by transiently transfecting the thus-transformed cell line with a third DNA construct containing a constitutive promoter capable of functioning in the cell line operably linked to the DNA molecule, and then culturing the thus-transfected cell line so that it can be assayed to determine the inhibitory activity of the DNA molecule. Antibodies specific to Sprm-1, and their use in diagnosing male germ cell Sprm-1-mediated diseases or conditions are further disclosed.