The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 04, 1997
Filed:
Apr. 10, 1995
Applicant:
Inventor:
C Bruce Bagwell, Topsham, ME (US);
Assignee:
Maine Medical Center Research Institute, Portland, ME (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 536 243 ;
Abstract
A fluorescent probe including (1) a nucleotide sequence which (a) has a segment complementary to the polynucleotide target and (b) is capable of forming one or more imperfect hairpins with at least one of the hairpins including the just-mentioned segment or a part thereof; and (2) at least one donor fluorophore and at least one acceptor fluorophore covalently attached to the nucleotide sequence so that only when one or more imperfect hairpins are formed, one of the donor fluorophores and one of the acceptor fluorophores are in close proximity to allow resonance energy transfer between them. Also disclosed is a method of using such a probe.