The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 25, 1997
Filed:
Jun. 10, 1994
Applicant:
Inventors:
Susan C Wright, Saratoga, CA (US);
James W Larrick, Woodside, CA (US);
Assignee:
Panorama Research, Inc., Mountain View, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C07K / ;
U.S. Cl.
CPC ...
435226 ; 435212 ; 435219 ; 530413 ;
Abstract
An apoptosis-associated protease having a relative mass of 24 kilodaltons and a defined amino acid composition is disclosed, together with a method for its purification from a cytoplasmic extract of mammalian cells treated with an apoptosis-inducing agent, such as tumor necrosis factor-.alpha. or UV irradiation, that comprises affinity chromatography with the serine protease inhibitor DK120 followed by heparin-sepharose chromatography. The protease has activity against the elastase-like substrate MAAPV and is capable of inducing apoptosis in isolated U937 cell target nuclei.