The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 28, 1997
Filed:
Mar. 09, 1995
Applicant:
Inventor:
Toru Murakami, Tokyo, JP;
Assignee:
NEC Corporation, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 25 ; 435 11 ; 436172 ;
Abstract
The invention provides a method for measuring the activity of cholesterol oxidase. First, a monolayer including sterol, a phospholipid and fluorescent dye labeled phospholipid is formed on a surface of a cholesterol oxidase aqueous solution. Then, it is observed whether sterol domain fade-out occurs in the monolayer. The activity of the cholesterol oxidase can be determined on the basis of both the period of time during which the sterol domain fade-out has occurred and a concentration of sterol in a cholesterol oxidase aqueous solution. This process can be applied to a method for estimating the toxicity of chemical compounds.