The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 21, 1997
Filed:
Jul. 06, 1994
Applicant:
Inventor:
Claudio Schneider, Udine, IT;
Assignee:
Talent S.r.l., Trieste, IT;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H / ;
U.S. Cl.
CPC ...
536 254 ; 536 2541 ; 564281 ;
Abstract
A process is disclosed for extracting genomic DNA from a biological sample containing a cell source comprising lysing the biological sample with a first cationic detergent in a solution of high salt concentration greater than 0.5M, under heating at 40.degree. to 80.degree. C., so as to solubilize nucleic acids and proteins present in the cell source. The lysed solution is then extracted with an organic solvent by leaving the DNA in the aqueous phase and precipitating any protein present in the aqueous phase. The DNA is then precipitated from the aqueous solution by diluting it at a salt concentration of below 0.5M and adding thereto a second cationic detergent.