The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 24, 1996
Filed:
May. 02, 1995
Douglas E Lewis, Oak Brook, IL (US);
Christine M Moore, Forest Park, IL (US);
United States Drug Testing Laboratories, Inc., Chicago, IL (US);
Abstract
A improved method for preparing a concentrated meconium extract containing basic and acid-neutral target analytes present in a meconium sample suspected of containing the target analytes is provided. The meconium sample is extracted with a mixed solvent of acetonitrile and a volatile organic acid and the resulting organic phase is separated. The organic phase is diluted with water to provide an organic solution which is passed through a mixed mode solid phase extraction column having strong cation exchange and hydrophobic functionality. Acid-neutral and basic target analytes are sequentially eluted from the column and the eluates are combined to form a combined extract. The combined extract is evaporated to dryness and reconstituted in buffer to provide a clean concentrated neonatal meconium extract for use in qualitative and quantitative analyses.