The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 10, 1996
Filed:
May. 04, 1995
Diana F Storhoff, Muncie, IN (US);
David Tabb, Greenfield, IN (US);
Boehringer Mannheim Corporation, Indianapolis, IN (US);
Abstract
A stable reagent for assaying an analyte from a fluid sample which includes an enzyme, a mediator, a tetrazolium salt and an oxidizing agent selected from the group consisting of sodium chlorate, 2,5-dimethylhexane-2,5-dihydroperoxide, benzoyl peroxide, t-butyl peroxide, sodium iodate, N-ethylmaleimide, t-butylperoxyacetate, nickel acetylacetonate, stannic chloride, rhodium (III) trichloride hydrate, and t-butylperbenzoate is described. Inclusion of an oxidizing agent from the group specified above stabilizes the reagent, thereby preventing a high blank reaction because a high blank introduces error into the assay of an analyte. The reagent may be provided in solution form by adding water, or may be included in a film by adding a film-forming agent. The reagent may be utilized to perform the assay of an analyte from a fluid sample. The assay may be conducted in a solution medium by adding the fluid sample to the liquid reagent, and spectrophotometrically measuring absorbance of the color indicating the presence of formazan which was produced by reduction of the tetrazolium salt under assay conditions. Alternatively, the assay may be conducted in a film medium by adding the fluid sample to the reagent incorporated in the film, and spectrophotometrically measuring reflectance or transmittance of the color indicating formazan.