The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 10, 1996
Filed:
Jun. 29, 1994
Christopher Bieniarz, Highland Park, IL (US);
Jeffrey B Huff, Park Ridge, IL (US);
Denis R Henrard, Lake Forest, IL (US);
Abbott Laboratories, Abbott Park, IL (US);
Abstract
Phenanthridium compounds are provided which are comprised of intercalators and substituted intercalator segments having a functionalized chain or chains which provide a high affinity for binding to the DNA molecule and show reduced self-quenching, while providing superior transport kinetics. The compounds have been found to provide enhanced fluorescence when bound to a DNA molecule within a fluorescent flow cytometry environment which is about eight to ten times brighter in fluorescence than 'bis' structure intercalators and other known intercalators utilized in flow cytometry environment, said enhancement of fluorescence allows a drastic reduction in the amount of intercalator needed for applications of similar sensitivity than those using known intercalators. Substantial improvements in material use efficiency, accuracy and dependability of various DNA studies are direct results of the inventive intercalator compounds, said compounds having use in known DNA intercalator applications inclusive of flow cytometry, gel electrophoresis, DNA detection, immunoassay for DNA, fluorescence in situ hybridization assays, DNA sequencing application, therapeutic application and conversion staining methodologies.