The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 03, 1996
Filed:
Jan. 21, 1994
Applicant:
Inventors:
John W Shultz, Verona, WI (US);
Douglas H White, Madison, WI (US);
Assignee:
Promega Corporation, Madison, WI (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; G01N / ; A61K / ;
U.S. Cl.
CPC ...
435 24 ; 435 23 ; 435 17 ; 435 15 ; 435 18 ; 435-4 ; 435 21 ; 435 16 ; 435810 ; 435975 ; 436-2 ; 436 12 ; 436 13 ; 436 14 ; 436 15 ; 436 16 ; 436 17 ; 436 63 ; 436 74 ; 436172 ; 436800 ; 530300 ;
Abstract
The present invention is directed to the assay and purification of proteins, and particularly to the non-radioactive assay and purification of protein kinases, phosphatases and protease by incubating the enzyme with a substrate modified peptide to form a product modified peptide under conditions where the enzyme is active. The product modified peptide and substrate modified peptide are then separated, and the product modified peptide is measured. The present invention is also directed to kits and bioreagents for performing the assays.