The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 1996

Filed:

Dec. 07, 1993
Applicant:
Inventors:

Toshikage Hiraoka, Asaka, JP;

Tetsuji Tanimoto, Tokyo, JP;

Yoshihiko Makino, Asaka, JP;

Tadashi Ninomiya, Tokyo, JP;

Naofumi Hora, Asaka, JP;

Yoshihiro Ashihara, Tokyo, JP;

Yukio Sudo, Asaka, JP;

Toshihiro Mori, Asaka, JP;

Assignees:

Fuji Photo Film Co., Ltd., Kanagawa, JP;

Fujirebio Inc., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ;
U.S. Cl.
CPC ...
435-79 ; 435-791 ; 435-792 ; 435 14 ; 435 18 ; 435 22 ; 435 25 ; 435 28 ; 435966 ; 435969 ; 435970 ; 436518 ; 436528 ; 436529 ; 436530 ; 436531 ; 436537 ; 436807 ; 436810 ; 422 56 ; 422 57 ;
Abstract

An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.


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