The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 22, 1996

Filed:

Aug. 12, 1992
Applicant:
Inventors:

James M Clark, San Mateo, CA (US);

Kevin R Shoemaker, San Francisco, CA (US);

Robert L Warne, San Francisco, CA (US);

Assignee:

Arris Pharmaceutical Corporation, South San Francisco, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435226 ; 435 691 ; 435 697 ; 435 698 ; 435 712 ; 4351723 ; 4353201 ; 4352402 ; 4352523 ; 43525233 ; 935 10 ; 935 14 ; 935 29 ; 935 47 ; 935 48 ; 935 70 ; 935 72 ; 935 73 ; 536 232 ;
Abstract

The instant invention is directed to recombinant production of functionally active chymase. 'Functionally active' as used herein refers to the ability to exhibit one or more functional activities of a full-length wild-type chymase protein. In a preferred aspect, a proteolytically inactive chymase fusion protein comprising a functionally active portion of a non-chymase protein joined to the amino-terminus of the chymase protein is produced, which, upon cleavage away of the non-chymase fusion protein portion, becomes proteolytically active. A refolding procedure for increasing yields of proteolytically active recombinant chymase is provided. The invention is further directed to use of the recombinant chymase thus produced for preparing chymase-specific antibodies.


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