The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 15, 1996

Filed:

May. 09, 1995
Applicant:
Inventors:

Minoru Ohashi, Tokyo, JP;

Osamu Adachi, Yamaguchi, JP;

Assignee:

Kikkoman Corporation, Chiba-ken, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12N / ; A23L / ;
U.S. Cl.
CPC ...
435 25 ; 435-4 ; 435 27 ; 435 28 ; 435183 ; 435191 ; 435192 ; 435225 ; 4352875 ; 435814 ; 435817 ; 435917 ; 4352871 ; 426 52 ; 426 56 ;
Abstract

The present invention relates to a method of determining histamine content as a freshness index of food. An examination liquid is injected in a reaction cell, an amount of dissolved oxygen (DO) is recorded through an oxygen sensor and an amplifier in the recorder. Then, an enzymatic reagent having histamine oxidase activity is injected in the reaction cell, a decrease in the dissolved oxygen is recorded in the recorder, and the histamine concentration is determined on the basis of the decrease by a micro computer.


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