The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 08, 1996

Filed:

May. 26, 1994
Applicant:
Inventors:

Kohji Yamamoto, Kyoto, JP;

Muneo Tokita, Nagaokakyo, JP;

Misako Inagami, Takatsuki, JP;

Sinichi Hirako, Nagaokakyo, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ;
U.S. Cl.
CPC ...
436 63 ; 436 10 ; 436164 ; 436172 ; 436800 ; 435-2 ; 435-6 ; 435 29 ; 435 34 ;
Abstract

The invention provides a method of quantitating reticulocytes with high sensitivity and high accuracy at low cost employing a fluorescent dye suitable for the staining of reticulocytes. A blood sample stained with a fluorescent dye composition containing TOTO-3, TO-PRO-3, POPO-3, PO-PRO-3, YOYO-1, YO-PRO-1, YOYO-3 or YO-PRO-3 as the fluorescent dye is exposed to light in the red-wave length region in the case of TOTO-3 or TO-PRO-3, light in the green region for POPO-3 or PO-PRO-3, light in the blue region for YOYO-1 or YO-PRO-1, or light in the orange region for YOYO-3 or YO-PRO-3 and the resultant fluorescent emission from the blood sample is measured to enumerate reticulocytes.


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