The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 24, 1996
Filed:
Nov. 23, 1994
Applicant:
Inventor:
Arne Lundin, Dalaro, SE;
Assignee:
Merck Patent GmbH, Darmstadt, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435-4 ; 435-8 ; 435 39 ; 435259 ; 435810 ; 436 17 ;
Abstract
A method of preparing an extract of an intracellular component, wherein cells are contacted with an extractant to generate an extract solution, which comprises contacting the solution with a cyclodextrin to neutralize the extractant. preferred extractants are cationic or other kinds of surfactants. The cyclodextrin is preferably used in solution. Preferred intracellular components are ATP which can, after neutralization of the extractant, be assayed by a firefly luciferase assay; and DNA or RNA which can, after neutralization of the surfactant, be amplified or further processed in other ways.