The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 03, 1996
Filed:
Apr. 09, 1993
Istv an Ott, Budapest, HU;
Tibor Klupp, Budapest, HU;
Istv an Moln ar, Budapest, HU;
Andr as Patthy, Budapest, HU;
Istv an Barta, Budapest, HU;
Zsuzsa Bark o n ee T oth, Budapest, HU;
G abor Ambrus, Budapest, HU;
J anos Sal at, Budapest, HU;
Anik o Tegdes, Budapest, HU;
Imre Moravcsik, Budapest, HU;
Cecilia Egy ud, Budapest, HU;
K arnly Albrecht, Budapest, HU;
K alm K oncz ol, Budapest, HU;
Attila Vincze, Budapest, HU;
Eva Barab as, Budapest, HU;
Gy orgy M at e, Budapest, HU;
Gy orgy Kiss, Szeged, HU;
P eter Kiss, Szeged, HU;
K alm P olya, Debrecen, HU;
J anos Erdei, Debrecen, HU;
Eva Guly as, Debrecen, HU;
Erika Zilahi, Debrecen, HU;
Biogal Gyogyszergyar Rt., Budapest, HU;
Abstract
The present invention relates to a process for producing recombinant desulphatohirudin by means of culturing microorganisms. Concerning the codon usage of microorganisms the synthesized nucleotide sequences were joined downstream of and in reading frame with isolated promoters and signal sequences, subsequently the expression/secretion cassettes comprising the foregoing elements were inserted into plasmid DNAs allowing the cultivation of cells under selective culture conditions. E. coli, Saccharomyces and Streptomyces species were transformed with the said recombinant plasmids to biosynthesize the thrombin inhibitor desulphatohirudin HV-1 which was then isolated and identified. The thus-produced desulphatohirudin can be used to inhibit blood coagulation.