The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 1996
Filed:
Sep. 23, 1992
David T Curiel, Chapel Hill, NC (US);
Max L Birnstiel, Vienna, AT;
Matthew Cotten, Vienna, AT;
Ernst Wagner, Langenzersdorf, AT;
Kurt Zatloukal, Vienna, AT;
Christian Plank, Vienna, AT;
Berndt Oberhauser, Vienna, AT;
Walter G Schmidt, Vienna, AT;
Boehringer Ingelheim International GmbH, , DE;
Genentech, Inc., San Francisco, CA (US);
Abstract
A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.