The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 1996
Filed:
Aug. 02, 1993
Applicant:
Inventors:
Myron F Goodman, LaCanada, CA (US);
Linda J Reha-Krantz, Edmonton, Alberta, CA;
Assignee:
Other;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ;
U.S. Cl.
CPC ...
435 911 ; 435 912 ; 536 2714 ; 536 282 ;
Abstract
There are provided variant family B DNA polymerases having no 3'.fwdarw.5' exonuclease activity. These variant polymerases have utility as DNA sequencing polymerases. Methods for DNA sequencing with family B DNA polymerases and chain-terminating nucleotides not previously used for sequencing have been developed. The methods disclosed involve the use of family B DNA polymerases not known heretofore to have utility in DNA sequencing, such as variant or wild type forms of phage T4 DNA polymerase or Escherichia coli DNA polymerase II, with novel combinations of deoxynucleotides and chain-terminating nucleotides.