The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 30, 1996

Filed:

Sep. 14, 1994
Applicant:
Inventors:

Kin-Ming Lo, Wellesley, MA (US);

Yukio Sudo, Lexington, MA (US);

Stephen D Gillies, Hingham, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435697 ; 435 698 ; 435 701 ; 4352401 ; 4352523 ; 4353201 ; 5303873 ; 5303911 ; 5303917 ; 530402 ; 530344 ; 530345 ; 536 231 ; 536 234 ; 536 2353 ;
Abstract

Disclosed are DNAs produced by recombinant techniques for inducing the expression and subsequent secretion of a target protein. The DNAs encode, in their 5' to 3' direction, a secretion cassette, including a signal sequence and an immunoglobulin Fc region, and a target protein. The DNAs can be transfected into a host cell for the expression, production and subsequent secretion of the target protein as a fusion protein. The secreted protein can be collected from the extracellular space, and further purified as desired. The secreted fusion protein additionally can be proteolytically cleaved to release the target protein from the secretion cassette.


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