The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 1996

Filed:

Mar. 12, 1993
Applicant:
Inventor:

James D Berg, 1390 Vollen, NO;

Assignee:

Other;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12N / ;
U.S. Cl.
CPC ...
435 34 ; 435-4 ; 435 29 ; 435 38 ; 435968 ; 4352521 ; 43525233 ;
Abstract

A rapid presence-absence method for determining if fecal coliform cells are present in a sample. Portions of the original sample are filtered and retained upon microporous filters which are placed in incubation containers having an actuating medium containing a fluorogenic substrate. The samples are incubated for predetermined durations of from about twenty minutes to six hours. After adjusting the pH is incubation to an alkaline level, the containers are irradiated and the fluorescent light emitted is measured. The measured values are adjusted for background fluorescence and corrected for extraneous sources of fluorescence. It is concluded that fecal cells are present in the original sample (i.e., at least one fecal coliform cell per 100 milliliters) when the corrected fluorescence values are positive and meet certain predetermined criteria.


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