The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 1996
Filed:
Aug. 25, 1993
David J Gibboni, Havertown, PA (US);
Viktor G Kartsev, Chernogolovka, RU;
Alexander G Ignotenko, Donetsk, UA;
Alexei Sukhotin, Chernogolovka, RU;
Actimed Laboratories, Inc., Burlington, NJ (US);
Abstract
A color-forming coupler is provided which can be used in test compositions, as well as methods and apparatus for detection of hydrogen peroxide. The color-forming coupler compounds react with a hydrazone or an aminoantypyrine in the presence of peroxidase and hydrogen peroxide to produce a darkly colored compound. In the presence of peroxidase and hydrogen peroxide, the couplers of the present invention react with a hydrazone or an aminoantipyrine to produce a deeply colored dye, which can be used to give a visual indication of the analyte present in the sample. These couplers can be used in diagnostic tests in which the analyte is converted to hydrogen peroxide. The color-forming couplers are N,N-disubstituted anilines of the formula shown in FIG. 1 , wherein R.sup.1 and/or R.sup.2 =H, C.sub.1 -C.sub.9 alkyl, C.sub.1 -C.sub.9 -C.sub.9 alkoxy, NR.sup.3 R.sup.4 (where R.sup.3 and/or R.sup.4 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroryl), F, Cl, Br, I, COOR.sup.5 (where R.sup.5 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl), CN, CONR.sup.6 R.sup.7 (where R.sup.6 and/or R.sup.7 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteraryl), aryl, aryloxy, heteroaryl, heteroaryloxy; and R.sup.11, R.sup.12, R.sup.13, and/or R.sup.14 =H, C.sub.1 -C.sub.9 alkyl, C.sub.1 -C.sub.9 alkoxy, or NR.sup.15 R.sup.16 wherein at least one of R.sup.11 and R.sup.14 is NR.sup.15 R.sup.16 wherein only one of R.sup.11 and R.sup.14 is H or C.sub.1 -C.sub.2 alkyl; where R.sup.15 and/or R.sup.16 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl, F, Cl, Br, I, COOR.sup.17, where R.sup.17 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl, CN, CONR.sup.18 R.sup.19 (where R.sup.18 and/or R.sup.19 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl, aryl, aryloxy, heteroaryl, heteroaryloxy; and n=0-10 and m=0-10 and Z and/or y=H, OH, SH, COOR.sup.8 (where R.sup.8 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl), CN, NR.sup.9 R.sup.10 (where R.sup.9 and/or R.sup.10 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl), NR.sup.20 NHR.sup.21 (where R.sup.20 and/or R.sup.21 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl), where at least one of Z or Y is COOR.sup.8, CN NR.sup.9 R.sup.10, or NR.sup.20 NHR.sup.21 or 4-amino antipyrines.