The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 1996
Filed:
Jul. 07, 1994
Henri Lichenstein, Ventura, CA (US);
Amgen Inc., Thousand Oaks, CA (US);
Abstract
The isolation and characterization of a novel polypeptide, designated Protease X, identified while screening for proteases exported into the media by Streptomyces lividans, is described [SEQ ID NO: 2]. Also disclosed is the DNA sequence of the gene encoding this protein [SEQ ID NO: 1]. The polypeptide was found to have proteolytic activity on the substrate succinyl-ala-ala-pro-phe-pNitroanilide, also cleaved by chymotrypsin, subtilisin, and cathepsin G. In addition, methods for purifying the protease and raising antibodies against it are reported. Lastly, the generation of an S. lividans strain deficient in this proteolytic activity is reported. Such a strain may prove useful as a host for production of heterologous protein products.